Pump
5110: Pressure up to 40 MPa
5160: Pressure up to 60 MPaBasic UHPLC models with a wide range of HPLC application

Item	5110	5160
Operating flow late range	0.001 to 9.999 mL/min	0.001 to 5.000 mL/min
Maximum operating pressure	40 MPa (0.001 to 5.000 mL/min) 20 MPa (5.001 to 9.999 mL/min)	60 MPa (0.001 to 2.500 mL/min) 30 MPa (2.501 to 5.000 mL/min)
Dimensions	340(W) × 440(D) × 140(H) mm
Number of mixed solvents*1	Up to 4

*With low pressure gradient unit (optional)
Autosampler
5260: Withstand pressure 60 MPa, Loop injection method
5280: Withstand pressure 60 MPa, Direct injection methodProduct lineup to accommodate various applications

Item	5260	5280
Sample injection system	Loop injection method (Cut injection, All volume injection, Full loop injection method)	Direct injection method
Withstand pressure	60 MPa	60 MPa
Washing function	Equipped with built-in washing pump Two-solvent washing function	Wash solvent delivery by a syringe
Sample rack temperature control	1 to 45 °C	1 to 35 °C
(Temperature setting range)	Capable of cooling and heating	Cooling only
Sample capacity	Standard	120 × 1.5 mL	200 × 1.5 mL
Optional	72 × 4 mL	128 × 4 mL

Column Oven
5310: Horizontal type
6310: Vertical typeEasily accommodates a 300-mm analytical column fitted with a guard-column

Item	5310	6310
Temperature setting range	1 to 85 °C (1 °C step)	4 to 90 °C (1 °C step)
Temperature control range	[Ambient temperature -15 °C] to [Ambient temperature +60 °C] and range of the temperature setting	[Ambient temperature -15 °C] to [Ambient temperature +75 °C] and within temperature setting range
Column capacity	300 mm × 3 (Maximum)
Built-in 3-liter water tank	-	Available
Dimensions	410(W) × 440(D) × 140(H) mm	165(W) × 515(D) × 689(H) mm (Legs are not included)

Detector
5410: UV Detector
5420: UV-Vis Detector
5430: Diode Array Detector
5440: Fluorescence Detector
5450: RI Detector
5610: MS Detector

Item	5410 UV Detector	5420 UV-Vis Detector	5430 Diode Array Detector
Optical system	Double-beam ratio photometric system	Single-beam ratio photometric system
Wavelength range	190 to 600 nm	190 to 900 nm	190 to 900 nm
Flow cell	Optical path length 10 mm
Simultaneous measurement	Two-wavelength*	spectrum acquisition
Dimensions	340(W) × 440(D) × 140(H) mm

*Please contact us for details.

Item	5440 Fluorescence Detector
Light source	Xe lamp, Hg lamp for checking wavelength
Wavelength range	Ex: 200 to 850 nm Em: 250 to 900 nm (Change photomultiplier at 731 nm or more)
Flow cell	Irradiation volume 12 µL
Dimensions	340 (W) × 440 (D) × 280 (H) mm

Item	5450 RI Detector
Refractive index range	1 to 1.75
Temperature control range	OFF, and 30 to 50 °C
Dimensions	340 (W) × 440 (D) × 140 (H) mm, excluding projections

Item	5610 MS Detector
Measurement mass range (m/z)	20 to 1,000
Ion source	Electrospray ionization (ESI)
Dimensions	440 (W) × 610(D) × 430 (H) mm
N2 gas usage	Max flow rate 3.4 L/min, Pressure 300 ± 20 kPa

Organizer

Item	Organizer
Output power	DC24 V, 450 W Supplies power to one pump, one autosampler, one detector (UV detector, UV-Vis detector, Diode array detector, or RI detector), and one interface control board
Bottle capacity and space	1.0 L bottle × 6 and 500 mL bottle × 3(Maximum), 314(W) × 280.8(D) mm
Dimensions	340 (W) × 420 (D) × 200 (H) mm

Data

System Performance
Analysis Example from the Chromaster 60 MPa System
The 60 MPa system, which can be used with columns containing 2.0 µm or smaller particles, and core-shell columns, is capable of conventional HPLC analysis and also ensures improved resolution performance and shorter analysis time.

Conditions

Sample	Alkylphenones
Column	LaChrom II C18 (5 µm) 4.6 mm I.D. × 150mmL LaChromUltra II C18 (1.9 µm) 2.0 mm I.D. × 50 mmL
Column temperature	40 °C
Mobile phase	A: H2O, B: CH3CN
Gradient mode	High Frequency Mode
Wavelength	247 nm

The following options are used with UHPLC column:

• Low dispersion piping kit

• Semi-micro mixer (200 µL)

• Semi-micro flow cell unit

Example of Carry-over analysis (5260)
Even when highly adsorbing Chlorhexidine is analyzed, the carry-over is extremely low.

Easy to use

Analysis Example from the Chromaster 60 MPa System

Most optional accessories are internally mounted to reduce the HPLC system's height. The handle located on the front side of the organizer moves vertically for easy access to solvent bottles.

Module operations and the replacement of consumable and maintenance parts can be performed from the front side.

With attention to detail on the housing of tubes and wires, the system keeps tubes from getting tangled up, ensures the ease of replacement, and provides adequate seismic stability.

Column management system (optional)

Hitachi's column management system can manage the Log information on analytical columns and guard columns from any manufacturer.
Log information can be written and read through a connector mounted on the column oven or USB port in the computer. ID Tags can be used repeatedly^*.

*Approximate read/write life time : 100,000 times

Wakeup (automatic pre-analysis tasks) and Sleep (automatic post-analysis tasks) programs

Automatic system Wakeup and Sleep from GUI

• In Conditioning, up to 10 programs can be created by combining any of the module settings, such as pre-analysis tasks of system (Wakeup), and post-analysis tasks of system (Sleep).
• For Wakeup program ending time, you can specify any time on current day, the following day, or two days later.
• The Sleep program starts at a specified time on the current day/the following day, or after the end of a continuous analysis run.

Automated system standby can reduce the amount of time required to make preparations for an analysis run.