iScope fluorescence
For biological and medical applications fluorescence microscopes are used to observe certain parts of living cells and tissues with help of fluorophores. One or more fluophores are added to the parts of the specimen to be observed. When the fluorophores are exposed to so-called exicitation light, the excitation energy is absorbed by the fluorophore that start emitting so-called emission light that can be observed by the microscope. One can distinguish tumor cells from other cells, follow biological processes or prove the presence or absence of antibodies, etc
The excellent optics and mechanical components make the iScope a state-of-the art microscope that is suitable for fluorescence applications in Life Science and Biotechnical fields
The iScope fluorescence microscopy can be supplied with:
• 6-position rotating attachment with 100 W mercury vapor light source
• 3-position slider attachment with 100 W mercury vapor light source
Product Details
6-positions rotating attachment
With 100 W mercury vapor light source
The 6-positions rotating fluorescence attachments are supplied with a standard 100 W HBO mercury vapor light source for broad powerful light spectrum. Supplied with standard fluorescence filters sets for excitation in the blue and green light spectrum and a UV protection shield
Some models are also available as in vitro diagnostic medical devices class A - (EU) 2017/746
Contact your Euromex dealer
3-positions slider attachment
With 100 W mercury vapor light source
The 3-positions slider fluorescence attachments are supplied with standard 100 W HBO mercury vapor light source for broad powerful light spectrum. Supplied with standard fluorescence filters sets for blue and green excitation and a UV protection shield
Some models are also available as in vitro diagnostic medical devices class A - (EU) 2017/746
Contact your Euromex dealer
- HWF 10x/22 mm eyepieces
- Binocular and trinocular models
- Optional ergonomic tilting head
- Quintuple revolving nosepiece
- E-plan, plan and plan Fluarex IOS corrected objectives
- 187/230 x 140 mm rackless stage with integrated X-Y stage
- Diascopic intensity adjustable 3 W NeoLED Köhler illumination
- Epi-fluorescence 100 W HBo mercury vapor
- Blue and Green LED fluorescence
- iCare sensor for energy saving
- State-of-art microscope
- Excellent optics
- Suitable for fluorescence applications
- CSS - Cable Storage System
- Provided with APL (Antimicrobial Protection Layer)
EYEPIECES
Extended EWF 10x/22 mm eyepieces, Ø 30 mm tubes. Eyepieces can be secured to the tubes. The trinocular head of the Plan infinity corrected models (PLi and PLFi) has a light path selector (100:0 / 50:50) and ± 5 diopter adjustments on both tubes.
The trinocular head of the E-Plan infinity corrected models (EPLi) has a fixed built-in light path beamsplitter (50:50) and ± 5 diopter adjustment on the left tube
HEAD
- Binocular and trinocular Siedentopf type heads with 30° inclined tubes. Interpupillary distance from 48 to 76 mm
- A unique rotating system allows the ergonomical positioning of both tubes in a high (431 mm) and in a low position (397 mm)
- The trinocular head has a 23.2 mm photo tube. This fits all C-mount adapters supplied with Euromex cameras
To order an iScope® with tilting head use reference IS.3158-xxx (Epli/Pli/PLFi)
To order an iScope® with tilting trino head use reference IS.3159-xxx (EPLi/Pli/PLFi)
NOSEPIECE
Revolving quintuple reversed nosepiece on ball bearings
OBJECTIVES
The EPLi and PLi objectives are suitable for Blue and Green standard filter sets and other filter sets in the visible spectrum.
The plan Fluarex PLFi ™ objectives are suitable for the entire spectrum including Ultra Violet and Violet
* optional
All optics are anti-fungus treated and anti-reflection coated for maximum light throughput
STAGE
187/230 x 140 mm rackless stage equipped with double slide holder and integrated 79 x 52 mm mechanical stage. The iScope® rackless stage has no protruding parts, enabling more smooth movements and is safer
CONDENSER FOR BRIGHTFIELD
The standard height adjustable Abbe N.A. 1.25 condenser for brightfield with magnification indication.
Also accepts sliders with phase annuli suitable for plan phase 10x/20x or S40x/ S100x oil immersion IOS objectives for economical phase contrast. A slider with a simple dark stop for darkfield up to 400x magnification is also available for this condensor
CONDENSER FOR ZERNIKE PHASE CONTRAST
An optional height adjustable Zernike NA. 1.25 phase contrast disc condenser with darkfield stop position for darkfield contrast observation up to 400x magnification and plan phase 10/20/S40x and S100x oil immersion PLPHi IOS objectives
FOCUSING
Coaxial coarse and fine adjustments, 200 graduations, 1 μm per graduation, 200 μm per rotation, total travel range is approximately 24 mm. Supplied with an adjustable rack stop to prevent damage to sample and objectives. The coarse adjustments are equipped with friction control
ILLUMINATION
3 W adjustable Köhler NeoLED diascopic illumination with internal 100-240 V power supply
The innovative NeoLED design offers larger apertures, allowing the optical system of the iScope microscope to produce images at higher resolutions, very close to the theoretical diffraction limit of the optics. Other benefits of the NeoLED is the low energy consumption, no heating and a long operating lifetime
The Köhler illumination on models with IOS plan and plan phase infinity corrected system provides high contrast and a maximum achievable resolving power of the optics. Also available for other models as an option
ILLUMINATION FOR FLUORESCENCE
The 6-position rotating and 3-position slider fluorescence attachments are supplied with:
- a 100 W HBO mercury vapor light source for broad powerful light spectrum. Including an 100-240 V external power supply with indication of the life span of the HBO bulb
- Standard fluorescence filters sets for- blue excitation (EX460- 490 (BP) DM500 EM520 (LP)
- green excitation (EX510-550 (BP) DM570 EM580 (LP) and a UV protection shield
The iScope® fluorescence microscopes equipped with a 100 W mercury vapor light source can be supplied withmore specific, optimized and application related fluorescence filters sets (see next pages)
LED ILLUMINATION FOR FLUORESCENCE
Two LED sources for fluorescence are available for 4 or 9 excitation wavelengths.
See our product LE.6400
STANDARD GENERAL PURPOSE FLUORESCENCE FILTER SETS
IS.9745 - Blue excitation filter set
EX: 460-490 nm bandpass DM: 500 nm EM: 520 nm longpass
IS.9746 - Green excitation filter set
EX: 510-550 nm bandpass DM: 570 nm EM: 580 nm longpass
IS.9748 - UV excitation filter set
EX: 330-385 nm bandpass DM: 400 nm EM: 420 nm longpass
IS.9747 - Violet excitation filter set
EX 400-410 nm bandpass DM 455 nm EM 455 nm longpass
ICARE SENSOR
The unique iCare Sensor is developed to avoid unnecessary loss of energy. The illumination of the microscope automatically switches off shortly after microscopists step away from their position
CSS - CABLE STORAGE SYSTEM
iScope allows users to easily insert the power cable into the back of the instrument, which enables easy storage. The integrated carrying grip at the back of the microscope ensures safe transportation of the microscope
CONFIGURATIONS
The iScope microscope is available in many configurations and can be upgraded with a variety of contrast types
PACKAGE CONTENT
Supplied with power cord, dust cover, a spare fuse, user manual and 5 ml immersion oil for models with S100x objective. Models with 100 W mercury vapor light source have a UV protection shield. All packed in polystyrene boxes
